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. 2021 Feb 2;11:549179. doi: 10.3389/fimmu.2020.549179

Figure 5.

Figure 5

P2RX1 is required for facilitating neutrophil’ activation and glycolytic metabolism in acute pancreatitis. (A) Neutrophils were isolated from pancreas of WT or P2RX1-KO mice at 8 h after the first administration of caerulein. Expressions of inflammatory cytokines and glycolytic metabolism genes were detected by RT-qPCR. 2− δδ Ct value was used for comparisons the fold change of mRNA expression to WT neutrophils. (B) Neutrophils were isolated from bone marrow of unstimulated WT or P2RX1-KO mice. Serum was used to stimulated neutrophils in the presence or absence of 2-DG for 6 h. Inflammatory cytokines were measured by RT-qPCR. 2− δδ Ct value was used for comparisons the fold change of mRNA expression to vehicle serum-treated WT neutrophils. (C) Neutrophils were isolated from bone marrow of WT or P2RX1-KO mice. After stimulation with AP mice serum for 6 h, extracellular acid ratio (ECAR) was measured. (D) Pancreas P2RX1 expression in NC mice or AP mice were determined by RT-qPCR. 2− δδ Ct value was used for comparisons of the fold change of mRNA expression to NC pancreas. (E) Neutrophils were isolated from bone marrow of WT or P2RX1-KO mice and stimulated with AP serum. Extracellular ATP was detected in indicated time points. (F) Neutrophils were isolated from bone marrow of WT or P2RX1-KO mice and stimulated with AP serum with or without apyrase. Inflammatory cytokines were measured by RT-qPCR. 2− δδ Ct value was used for comparisons of the fold change of mRNA expression. Statistics were calculated using Student’s t test (A, E, F), or one-way ANOVA with Tukey post-tests (B, C). *P < 0.05, **P < 0.01, ***p < 0.001.