Figure 1.

Detection of the polymorphonuclear neutrophil (PMN) and bacterial load in the brain after infected with SS2. (A) Pathological changes in brain tissue were observed by HE staining. The scale bar represents 100 μm (top), 400 μm (bottom). (B, C) Immunohistochemistry was used to observe the pathological changes in the brain tissues of infected mice. The scale bar represents 100 μm (top), 400 μm (bottom). (D) The infiltration of neutrophils in the brain homogenates of mice (n=5) was detected by flow cytometry. (E) The expression of ribosomal protein SA (RPSA) on the surface of neutrophils in brain homogenates was detected by flow cytometry. (F) Brain tissue bacterial load. Five mice were used in each group for each experiment. *p < 0.05, **p < 0.01, ***p < 0.001 compare with control group, ns, no significant difference.