Table 2.

Summary of the main published clinical evidence on 1PN-derived embryos. PN pronuclei, D3 day 3, STRs short tandem repeats, aCGH array comparative genomic hybridization, qPCR quantitative polymerase chain reaction, SNP single nucleotide polymorphism, NGS next-generation sequencing, TLM mite lapse microscopy, SET single embryo transfer, LBR live birth rate

Paper	Size	Workflow	PN assessment	Euploidy rate	LBR
Destouni 2018	268 cycles 132 1PN out of 2337 zygotes (6%) 15 D3 embryos out of 132 1PN zygotes (11%)	- ICSI - D3 biopsy - Blastocyst vitrification - Genome-wide haplotyping	Standard	Euploid-diploid biparental: 5 out of 14 D3 embryos (36%)	1 out of 1 euploid-diploid blastocyst SET (100%)
Mateo 2017	64 cycles 115 1PN-derived D3 embryos	- ICSI - D3 biopsy - Blastocyst vitrification - aCGH	TLM	Euploid: 15 out of 88 D3 embryos (17%)	1 out of 2 euploid blastocyst SETs (50%)
Capalbo 2017	678 cycles 200 1PN out of 3785 zygotes (5%) 13 blastocysts out of 200 1PN zygotes (6.5%)	- ICSI - TE biopsy - Blastocyst vitrification - qPCR (for aneuploidies) plus SNP-array/NGS (for ploidy)	Standard/TLM	Euploid-diploid: 3 out of 13 blastocysts (23%)	1 out of 2 euploid-diploid blastocyst SETs (50%)
Bradley 2017	4272 cycles 1192 1PN zygotes 106 blastocysts out of 1192 1PN zygotes (9%)	- IVF/ICSI - TE biopsy - Blastocyst vitrification - aCGH/NGS (for aneuploidies) plus STRs analysis among female balanced blastocysts (for ploidy)	Standard	Euploid-diploid: 55 out of 106 blastocysts (52%) [4 female blastocysts were not tested for ploidy due to absence of parental controls]	9 out of 26 euploid-diploid blastocyst SETs (35%)
Hondo 2019	11,588 cycles	- IVF/ICSI - Blastocyst culture	Standard	-	14 out of 73 untested blastocyst SETs (19%)