Table 2.

Independent variables and results obtained under different conditions based on 2-factor central composite design for response surface analysis

Run	Independent variables		Responses*
	F1	F2	pH	BI	TEAC	ORAC	DPPH	HMF	AC
	Temp (°C)	Time (min)		Abs	μmol TE/g	μmol TE/g	%	ppm	ppm
1	76	180	7.77 ± 0.02	0.91 ± 0.02	0.32 ± 0.01	0.73 ± 0.01	35.50 ± 0.05	0.27 ± 0.01	0.96 ± 0.12
2	100	180	7.25 ± 0.23	1.86 ± 0.04	1.12 ± 0.14	7.83 ± 0.24	84.96 ± 0.06	ND	337.39 ± 12.52
3	92	300	6.95 ± 0.06	1.94 ± 0.01	1.27 ± 0.02	8.20 ± 0.20	66.93 ± 0.15	ND	140.88 ± 3.06
4	60	60	8.36 ± 0.01	0.05 ± 0.01	0.78 ± 0.03	0.25 ± 0.01	4.08 ± 0.04	0.45 ± 0.10	ND
5	60	300	8.29 ± 0.02	0.24 ± 0.01	0.92 ± 0.15	0.68 ± 0.01	4.32 ± 0.02	0.22 ± 0.02	ND
6	76	180	7.77 ± 0.02	1.68 ± 0.01	0.32 ± 0.01	0.33 ± 0.02	35.50 ± 0.0.05	0.27 ± 0.01	ND
7	76	180	7.92 ± 0.03	1.68 ± 0.01	0.89 ± 0.02	0.72 ± 0.02	25.08 ± 0.16	0.17 ± 0.01	0.96 ± 0.12
8	76	10	8.35 ± 0.01	0.04 ± 0.01	0.10 ± 0.08	0.35 ± 0.01	3.30 ± 0.02	0.28 ± 0.02	ND
9	92	60	7.55 ± 0.56	1.59 ± 0.01	0.87 ± 0.04	1.33 ± 0.15	24.40 ± 0.02	ND	11.10 ± 0.05
10	53	180	7.86 ± 0.64	0.04 ± 0.01	0.80 ± 0.19	0.28 ± 0.01	2.79 ± 0.04	2.82 ± 0.12	ND
11	76	350	7.50 ± 0.29	1.93 ± 0.07	1.05 ± 0.01	1.88 ± 0.08	40.30 ± 0.18	0.13 ± 0.01	3.67 ± 0.50
12	76	180	7.92 ± 0.03	1.68 ± 0.01	0.89 ± 0.02	0.73 ± 0.01	25.08 ± 0.16	0.17 ± 0.01	0.96 ± 0.12
13	76	180	7.92 ± 0.03	0.91 ± 0.02	0.89 ± 0.02	0.72 ± 0.02	25.08 ± 0.16	0.17 ± 0.01	0.96 ± 0.12

*All results are the mean ± SD (n = 3)

ND not detected