Table 5.

Predictive values of sensitivity and specificity estimates for the probability of obtaining the best quality embryo develops from mature oocytes

	AUC (95% population limit)	S.E.M	p value*	Cut-off point**	Sensitivity	Specificity
miR-663b	0.87 (0.80; 0.94)	0.036	0.002	≥ 0.64	88.3	80
miR-320a	0.89 (0.83; 0.96)	0.032	0.001	≥ 0.79	75.0	92.5
miR-766-3p	0.86 (0.79; 0.94)	0.037	0.001	≥ 0.57	93.3	72.5
miR-132-3p	0.88 (0.82; 0.94)	0.032	0.003	≥ 0.59	95	72.5
miR-16-5p	0.85 (0.78; 0.93)	0.039	0.001	≥ 0.68	88.8	80.5
Combination of ff miR-663, 320a, 766-3p, 132-3p, and 16-5p	0.89 (0.71; 0.93)	0.034	0.001	–	87	83
ROS	0.65 (0.54; 0.76)	0.055	0.021	1.88	63.3	70
TAC	0.85 (0.77; 0.93)	0.049	0.001	0.89	90.2	80.3
LPO	0.83 (0.75; 0.91)	0.042	0.001	0.78	91.7	72.5
TBARS	0.69 (0.58; 0.80)	0.052	0.003	0.71	80	40
8-OHdG	0.75 (0.65; 0.85)	0.058	0.001	0.75	93.3	60.1
Combination of ff evaluated oxidative stress markers	0.78 (0.69; 0.85)	0.045	0.001	–	83.2	78.36
cfDNA	0.76 (0.67; 0.85)	0.047	0.001	0.61	82.4	66.7
Melatonin	0.85 (0.78; 0.92)	0.037	0.001	0.80	90.7	72.6

*The null hypothesis was true area = 0.5, after the adjustment of a number of attempts and the number of embryos. p values in italics considered statistically significant p < 0.05

**Estimated cut points that maximize sensitivity and specificity for observed range predictors