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. 2021 Feb 15;116(1):11. doi: 10.1007/s00395-021-00850-2

Fig. 3.

Fig. 3

β-AR activation in rabbit myocytes induces nuclear accumulation of HDAC4 and requires S265/266. a HDAC4 sequence with selected domain and residues indicated. NLS = nuclear localization sequence, NES = nuclear export signal. b Average values of HDAC4 nucleo-to-cytoplasmic fluorescence ratio of endogenous HDAC4 or cells infected with HDAC4-GFP (polled data) and treated for 30–60 min with either 1 μM Iso or 10 μM forskolin at 37 °C. c Average values of HDAC4 nucleo-to-cytoplasmic fluorescence ratio of cells infected with HDAC4-S265/266A-GFP and treated as in (b). d Dose-dependent accumulation of HDAC4 in the nucleus upon Iso treatment. Each point represents an n = 22–26. e Time course of HDAC4 redistribution in response to Iso (black) or forskolin (grey). Each point represents an n = 20–25. Representative example (f) and quantification (g) of immunoprecipitation with a 14–3-3 antibody and a GFP antibody to detect the HDAC4–14–3-3 complex with or without forskolin treatment. ***p < 0.0001