Fig. 8.
HDAC4 localization in human ventricular myocytes is regulated in similar manner. a Representative image of endogenous HDAC4 localization in cardiomyocytes isolated from non-failing human heart, detected by ICC. Bottom panel represent zoom-in of corresponding cell nuclei. b Average values of HDAC4 FNuc/FCyto ratio in non-failing (NF) human myocytes in the absence or presence of specific agonists or antagonists of PKA or CaMKII signaling. c Representative image of endogenous HDAC4 localization in cardiomyocytes isolated from failing human heart, detected by ICC. Bottom panel represent zoom-in of corresponding cell nuclei. d Average values of HDAC4 FNuc/FCyto ratio in failing human myocytes in the absence or presence of specific agonists or antagonists of PKA or CaMKII signaling. Cells were incubated with indicated drugs for 1 h at 37 °C. ***p < 0.0001, *p < 0.01