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. 2021 Feb 2;9:627976. doi: 10.3389/fcell.2021.627976

FIGURE 3.

FIGURE 3

The depletion of phagocytic immune cells influences survival after bacterial challenge. Bacterial challenge assays were performed in flies (A) or mosquitoes (B) following treatment with control (LP)- or clodronate liposomes (CLD). Survivorship was monitored in every day over an 8-day period to evaluate the effects of S. marcescens or S. aureus challenge. For mosquito challenge experiments (B), an additional control was added in which LP-treated mosquitoes were challenged with the injection of sterile PBS. Error bars represent the mean ± SEM of three independent replicates for Drosophila (20 per replicate) and Ae. aegypti (30 per replicate). Data were analyzed using a log-rank (Mantel-Cox) test using GraphPad Prism 6.0. Fly and mosquito images were created with BioRender.com.