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. 2020 Dec 22;162(3):328–338. doi: 10.1111/imm.13288

Figure 3.

Figure 3

AAV8‐mediated overexpression of CD1d leads to protection against pathological damage in S. japonicum‐infected mice. (A) Experimental design for AAV8 vector (AAV‐CD1d or AAV‐Ctrl) injection, cercariae infection and further analysis. (B–C) Effect of AAV vectors on CD1d protein expression in livers (B) and spleens (C) of mice injected with AAV‐CD1d or AAV‐Ctrl was analysed by Western blot. The graphs show the quantification of the Western blot. (D) Representative liver sections of S. japonicum‐infected mice injected with AAV‐CD1d or AAV‐Ctrl were H&E‐stained to show granulomas (original magnifications, ×100). (E) Granuloma areas with a single egg were measured. (F) Levels of alanine transaminase (ALT) and aspartate transaminase (AST) in the serum of mice with injection of AAV‐CD1d or AAV‐Ctrl were detected by an automatic biochemical analyser. Data are means ±SD from 5 mice per group and representative of two independent experiments. *P < 0.05, **P < 0.01 and ***P < 0.001; ns, not significant.