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. 2020 Dec 13;162(3):314–327. doi: 10.1111/imm.13286

Figure 5.

Figure 5

Human antibodies binding HCMV AD‐2 epitope preferentially use kappa (κ) light chains. (A) ELISA to determine light chain usage of Ig's binding to gB and AD‐2 epitope of HCMV human serum (n = 24). Horizontal dotted lines representing positive/negative cut‐off values for negative gB and negative AD‐2 samples were based on the IgG gB‐negative samples (n = 4). Statistical differences comparing the mean OD450 nm values for kappa (κ) and lambda (λ) chain use at each dilution were obtained from the Mann–Whitney test (ns, not significant; ****P < 0.0001). (B) ELISA data displaying comparative κ and λ usage in Ig's binding to gB and AD‐2 epitope of HCMV in serum samples. Horizontal and vertical and dotted lines representing positive/negative cut‐off values for negative gB and negative AD‐2 samples, respectively, were based on the IgG gB‐negative samples (n = 4). Correlation coefficients (Spearman's) and statistical significance for each set of determinations comparing κ or λ usage in Ig's binding to gB and AD‐2 epitope are displayed above each graph