Fig. 1. in-vivo studies and data extraction.
a Illustration presenting an overall schematic representation of the in-vivo study performed on male Wistar rats for 21 months28. The proteins from the AC and BA samples were identified by proteomic analysis and designated as L1 and L2, respectively. b The AR composition was identified by mass spectroscopy techniques. The AR constituent metabolites, thus identified were used to search structurally similar drugs in the DrugBank database. Further, the targets corresponding to the highest-ranked drug molecules were identified to relate their activity against each metabolite (L3). The intersection of L1, L2, and L3 had 40 proteins (L4). These proteins represent the set of proteins targeted by various AR metabolites, which leads to the drug action of the AR concoction. Therefore, we used list L4 for our further analysis.