Fig. 4.

Biocompatibility and in vitro cytotoxicity of brush copolymers and pure polymer. a Percentage cell viability obtained through MTT assay measurements of the indicated specimens. b Fluorescence images of cells treated with indicated systems after 24 h of incubation. c Phase-contrast images of HeLa cells grown on indicated polymeric films, the images of cells adhered on the sample surfaces (cell adhesion). d Optical density of the adhered cell for an indication of cell viability. e In vitro cytotoxicity by using pure drug and drug-loaded brushes in HeLa cells at 250 μg/ml of drug concentration at different time interval. f AO/EB stained fluorescence images of cells after treatment with pure drug and drug-loaded brush copolymers: Results are presented in mean value ± SD, n = 5. ***p < 0.001, **p < 0.01, and *p < 0.05. g In vitro cytotoxicity potential of pure drug and low graft density brush copolymer (D-P-L at 250 μg/ml of drug concentration) in B16-F10 melanoma cells at different time interval (incubation time)