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. 2021 Feb 2;11:604913. doi: 10.3389/fimmu.2020.604913

Figure 2.

Figure 2

IFNγ ELISpot and tetramer responses. (A, B) IFNγ-secreting spot forming cells (SFC) in BAL, lung, TBLN and PBMC in outbred (black boxes) and inbred (red circles) pigs were enumerated after stimulation with H1N1pdm09 or medium control. (B) The mean percentages for each population are shown. DPI 1 to 7, 9, 11, and 13 each show results from two outbred and two inbred pigs. DPI 6, 7, 13, 14, 20, and 21 also include results from three additional inbred pigs. (C, D) Proportions of NP290-298 CD8 T cells in tissues from Babraham pigs. Background staining with SLA matched tetramers containing irrelevant peptide has been subtracted. Dotted lines indicate proportions of tetramer positive cells in uninfected animals. Data from 2 outbred and 2 inbred pigs is shown for days 1 to 5 and 9 to 11. DPI 6, 7, 13, 14, 20, and 21 show data from 3 additional inbred pigs. Data in C and D is from 2 pigs (DPI 1–5, 9, 11), 3 pigs (DPI 14, 20, 21) or 5 pigs (DPI 6, 7, and 13).