Table 1.
Antibodies used.
| Antigen | Clone | Isotype | Fluorochrome | Source of primary Ab | Details of secondary Ab |
|---|---|---|---|---|---|
| Staining for conventional T cells | |||||
| CD4 | 74-12-4 | IgG2b | PerCP-Cy5.5 | BD Biosciences | |
| CD8β | PPT23 | IgG1 | FITC | Bio-Rad Laboratories | |
| TNF | MAb11 | IgG1 | BV421 | BioLegend | |
| IFNγ | P2G10 | IgG1 | APC | BD Biosciences | |
| IL-2 | A150D 3F1 2H2 | IgG2a | PE-Cy7 | ThermoFisher | rat-anti-mouse, IgG2a, BioLegend |
| Staining for γδ T cells | |||||
| TCR γδ | PGBL22A | IgG1 | PE-Cy7 | Cambridge bioscience | rat-anti-mouse, IgG1, BioLegend |
| CD8α | 76-2-11 | IgG2a | FITC | BD Biosciences | |
| CD2 | MSA4 | IgG2a | PerCP-Cy5.5 | Cambridge bioscience | rat-anti-mouse, IgG2a, BioLegend |
| TNF | MAb11 | IgG1 | BV421 | BioLegend | |
| IFNγ | P2G10 | IgG1 | APC | BD Biosciences | |
| IL-17A | SCPL1362 | IgG1 | PE | BD Biosciences | |