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. 2021 Feb 15;12:1034. doi: 10.1038/s41467-021-21337-7

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a PrimeDesign takes a single sequence as input encoding both the original reference and desired edited sequences, b recommends a candidate pegRNA and ngRNA combination to install the edit of interest, c provides sequence visualizations of the edit of interest, selected pegRNA and ngRNA designs, and predicted pegRNA secondary structures, and d enables the interactive design of both pegRNAs and ngRNAs that can be downloaded as a summary table.