TABLE 1.

Computational alanine scanning results for Rac1-Tiam1 complex.

Mutant	Domain	ΔΔG a (kcal/mol)
E31A	Switch 1	−4.9 ± 1.6
Y32A	Switch 1	−8.6 ± 0.8
D38A	Switch 1	−6.5 ± 0.5
N39A	Switch 1	−6.7 ± 0.5
Y64A	Switch 2	−6.0 ± 0.5
D65A	Switch 2	−4.3 ± 1.5
W56A	Close to switch 2	−12.6 ± 0.6

^aΔΔG = ΔG (wild type) − ΔG (mutant).