FIGURE 4.

Alginate-α-CGRP microcapsules delivery in two-day post-TAC mice reduces TAC-induced cardiac hypertrophy and fibrosis. (A) Representative images showing the size of the hearts after 28 days of delivery of alginate-α-CGRP microcapsules. (B,C) Bar diagrams showing the ratio of wet heart weight/tibia length, and wet lung weight/tibia length. (D) The paraffin-embedded LV sections were stained with H&E, WGA stain, and trichrome-collagen stain. Scale bar, 100 μm. WGA stained sections were used to measure cardiomyocyte size in LVs by the NIH-ImageJ software and plotted (E). LV collagen content, an indicator of fibrosis, was quantitated by the NIH-ImageJ software and plotted (F). Values were expressed as the mean ± SEM. *p < 0.05, TAC vs sham; **p < 0.05, TAC-alginate-α-CGRP vs TAC; #p > 0.05, TAC-alginate-α-CGRP vs sham; ns, non-significant compared to sham.