FIGURE 2.

BMP9 reduces HFD-induced obesity in mice (Figure 2C Increasing body weight was changed as weight gain). (A) Experimental flow chart. Male mice were fed an HFD (containing 60% fat) for 8 weeks and administered PBS or recombinant BMP9 200 ng/mice/day via intraperitoneal injection for another 4 weeks. (B) Representative images of mouse in different groups. (C) Body weight was measured at different timepoints. Weight gain was measured from week 0–12. (D) Food intake monitored over the last month. (E) The appearance and weight of VAT. (F) HE staining of VAT (original magnification, ×200) and adipocyte numbers/HMF of VAT in different groups. HMF: High magnification field. The data are presented as the means ± SEM. Control, n = 6; HFD and HFD+BMP9, n = 8 respectively. *p < 0.05, ****p < 0.0001.