Figure 1.

Apatinib inhibits cell viability and promotes ferroptosis in HCT116 cells. (A) The chemical structural formula of Apatinib. (B) Cell viability of HCT116 cells after treatment with apatinib (5, 10 and 20 μM) was determined using a cell counting kit-8 assay kit. The relative concentrations of (C) total iron and (D) Fe²⁺ were measured using commercially available kits, respectively. (E) The level of ROS was detected by a ROS assay kit using 2, 7-dichlorofluorescein diacetate (DCFH-DA, Invitrogen, USA). The levels of (F) MDA, (G) GSH and (H) GPx were evaluated using commercial kits. *P<0.05, **P<0.01 and ***P<0.001 vs control.

Abbreviations: ROS, reactive oxygen species; MDA, malondialdehyde; GSH, glutathione; GPx, glutathione peroxidase.