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. 2021 Feb 11;14:979–988. doi: 10.2147/OTT.S289722

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© 2021 Wang et al.

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DAB2IP regulates the phosphorylation of AKT and ERK in GC cells. (A) Western blot analysis of the indicated proteins in AGS and SGC7901 cells stably transfected with either control shRNA (NC) or DAB2IP shRNA (KD). Tubulin as a loading control. The bands were quantified and presented as the mean ± SEM. (B) Western blot analysis of the indicated proteins in BGC823 and MKN45 cells stably transfected with either empty vector (VEC) or plasmids encoding human DAB2IP (OE). Tubulin as a loading control. The bands were quantified and presented as the mean ± SEM. (C) Colony formation assay of SGC7901 cells (NC vs KD) with or without treatment of Ly294002 (20 μM) or U0126 (20 μM). The bands were quantified and presented as the mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001.