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. 2021 Feb 2;11:593633. doi: 10.3389/fphar.2020.593633

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Copyright © 2021 Yue, Yu, Wang, Liu, Bai and Yang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Anthocyanin represses high glucose-induced abnormal upregulation of interleukin (IL)-17 expression in cardiac fibroblasts isolated from neonatal mice. (A) Alterations in viability at 24 h. (B) Alterations in viability at 48 h. (C) Relative expression of IL-17 mRNA detected using quantitative reverse transcription PCR (qRT-PCR). (D) Expression of IL-17 protein. GAPDH served as an internal control. (E) Immunofluorescence staining of IL-17. Scale bar, 20 μm. ^p < 0.05 vs. Control, ^^p < 0.01 vs. Control, ^^^p < 0.001 vs. Control, ***p < 0.001 vs. NG, ###p < 0.001 vs. HG. NG, normal glucose (5.5 mM); HG, high glucose (25 mM); HG + AC, high glucose (25 mM) + anthocyanin (250 μg/ml); n = 5 for A-C, n = 3 for D-F.