Table 1.

Agilent 7890A GC and Autosampler Parameters.

GC Inlet
Mode	Pulsed Splitless
Temperature (°C)	235
Total Flow (mL/min Helium)	54
Septum Purge Flow (mL/min Helium)	3
Injection Pulse Pressure (psi until 1 min)	25
Purge flow to split vent (mL/min Helium at 1.1 min)	50
Gas saver (mL/min Helium after 3 min)	15
GC Program
Initial GC Oven Temperature (°C)	30
Initial Oven Hold Time (min)	1
Rate (°C/min)	75
Final GC Oven Temperature (°C)	300
Final Over Hold Time (min)	1.4
Run Time (min)	6
Equilibration Time (min)	0.1
GC Transfer Line (°C)	300
Oven Max Temperature (°C)	340
GC Column
LTM Column	DB–5 ms, 15 m × 250 μm × 0.25 μm
Head Pressure (psi)	2.14
Flow (mL/min Helium)	1
Average Velocity (cm/sec)	47
Holdup Time (min)	0.5
GC Autosampler
Syringe Size (μL)	10
Injection Volume (μL)	1
Post Washes A	3 with ethanol
Post Washes B	3 with acetone
Samples Washes	1
Sample Wash Volume (μL)	4
Samples Pumps	3
Viscosity Delay (sec)	3
Air Gap (μL)	0.2
GC Autosampler Barcode Mixer
Mixer	Enabled
Mixer Cycle	1
Mixer Time (sec)	10
Mixer Speed (rpm)	2000