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Detection of influenza A virus, influenza B virus, and SARS-CoV-2 based on RT-RPA and CRISPR-Cas12a assay. Schematic representation of specific primers and crRNAs for (a) M gene of influenza A virus, (b) M gene of influenza B virus, and (c) spike gene of SARS-CoV-2. (d) The workflow of the overall process for rapid detection based on RT-RPA and CRISPR-Cas12a assay within 1 h. (A color version of this figure is available in the online journal.)
