Table 1.
Oligonucleotides used for qRT-PCR, RT-RPA, and crRNA production.
| Assay | Primer name | Sequence (5′ ⟶ 3′) | Product size (bp) | Ref. |
|---|---|---|---|---|
| RT-RPA | SARS2_spike1-F | CCACTGAGAAGTCTAACATAATAAGAGGCTG | 201 | This study |
| SARS2_spike1-R | AATAAACTCTGAACTCACTTTCCATCCAACT | |||
| SARS2_spike2-F | AATCTATCAGGCCGGTAGCACACCTTGTAAT | 166 | ||
| SARS2_spike2-R | TCCACAAACAGTTGCTGGTGCATGTAGAAGTT | |||
| FluA-M-RPA-F | ACCGAGGTCGAAACGTATGTTCTCTCTATC | 219 | ||
| FluA-M-RPA-R | TCTACGCTGCAGTCCTCGCTCACTGGGCAC | |||
| FluB_M-RPA-F | CACAATTGCCTACYTGCTTTCATTRACAGAAGA | 168 | ||
| FluB_M-RPA-R | GCACCAATTAGTGCTTTCTGTATATCAGTTAAG | |||
| GAPDH_RPA-F | ACCAGGTGGTCTCCTCTGACTTCAACAGCG | 178 | ||
| GAPDH_RPA-R | ATGGCCCACATGGCCTCCAAGGAGTAAGAC | |||
| qRT-PCR | SARS-CoV2-N-F | CACATTGGCACCCGCAATC | 128 | 7 |
| SARS-CoV2-N-R | GAGGAACGAGAAGAGGCTTG | |||
| SARS-CoV2-N-P | FAM-ACTTCCTCAAGGAACAACATTGCCA-BBQ | |||
| SARS-CoV-RdRp-F | GTGARATGGTCATGTGTGGCGG | 100 | 7 | |
| SARS-CoV-RdRp-R | CARATGTTAAASACACTATTAGCATA | |||
| SARS-CoV-RdRp-P-Pan | FAM-CCAGGTGGWACRTCATCMGGTGATGC-BBQ | |||
| SARS-CoV2-RdRp-P | FAM-CAGGTGGAACCTCATCAGGAGATGC-BBQ | |||
| SARS-CoV2-E-F | ACAGGTACGTTAATAGTTAATAGCGT | 113 | 7 | |
| SARS-CoV2-E-R | ATATTGCAGCAGTACGCACACA | |||
| SARS-CoV2-E-P | FAM-ACACTAGCCATCCTTACTGCGCTTCG-BBQ | |||
| FluA_M_F | CATGGARTGGCTAAAGACAAGACC | 126 | 6 | |
| FluA_M_R | AGGGCATTTTGGACAAAKCGTCTA | |||
| FluA_M_P | FAM-ACGC T CACCG T GCCC A GT-BHQ1 | |||
| FluB_MP_F | CTCTGTGCTTTRTGCGARAAAC | 233 | ||
| FluB_MP_R | CCTTCYCCATTCTTTTGACTTGC | |||
| FluB_M_P | Cy5-TCAG C A A T GA A C ACAGCAA-BHQ3 | |||
| crRNA | SARS2-S1 | UAAUUUCUACUAAGUGUAGAUGAUUCGAAGACCCAGUCCCU | This study | |
| SARS2-S2 | UAAUUUCUACUAAGUGUAGAUCAAUCAUAUGGUUUCCAACC | |||
| FluA-M | UAAUUUCUACUAAGUGUAGAUGCCAUUCCAUGAGAGCCUCA | |||
| FluB-M | UAAUUUCUACUAAGUGUAGAUACCUAGACUCAGCCUUGGAA | |||
| GAPDH | UAAUUUCUACUAAGUGUAGAUCUGGUAUGACAACGAAUUUG |
Note: Bold nucleotide represents LNA residue; Underline sequence represents spacer sequence.