Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Nov 11;246(4):371–379. doi: 10.1177/1535370220966253

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2020 by the Society for Experimental Biology and Medicine

PMC Copyright notice

Figure 4. — IGF2-AS directly bins to DNMT1 and enhances the occupation of DNMT1 on IGF2 promoter. (a) qRT-PCR analysis of the location of IGF2-AS1 in MCF-7 and T47D cells. U6 and GAPDH were used as nucleus and cytoplasmic reference fractions, respectively. (b) qRT-PCR analysis of IGF2 expression in MCF-7 and T47D cells after DNMT1, DNMT3a, and DNMT3b overexpression. (c) The interaction possibility between IGF2-AS and DNMT1 predicted by RPISeq online tool. (d, e) RNA pull-down and RIP assays confirming the interaction between IGF2-AS and DNMT1 using biotinylated IGF2-AS probe and DNMT1 antibody, respectively. (f) ChIP assay analyzing the occupation of DNMT1 on IGF2 promoter in MCF-7 and T47D cells after IGF2-AS overexpression. (g) qRT-PCR analysis of IGF2 expression in IGF2-AS-overexpressing cells after DNMT1 knockdown. **P < 0.01.