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. 2021 Feb 9;12(1):e03374-20. doi: 10.1128/mBio.03374-20

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Copyright © 2021 Molgora et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

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FIG 5 — The loss of heparan sulfate on CHO cells significantly decreases adherence of TvAD1-add back parasite but not TvAD1-KO adherence. TvAD1-KO (A) and TvAD1-add back parasites exogenously expressing TvAD1 in the knockout background (B) were measured for their adherence to wild-type (WT), heparan sulfate-deficient (ΔHS), and GAG-deficient (ΔGAG) CHO cell monolayers. (C) Comparison of TvAD1-KO and TvAD1-add back parasite adherence to CHO cell monolayers. Data shown are means of independent experiments done in triplicate ± SEM. Statistical significance for A and B was determined using a one-way ANOVA with Tukey’s multiple-comparison test and unpaired t test for C.