FIG 6.

Measuring interaction between TvAD1 and heparan sulfate by ITC. Binding interaction between TvAD1 protein (146.2 μM) and heparan sulfate (HS) (1 mg/ml) was measured using ITC at 25°C. Initial injection was 0.2 μl of HS followed by 19 injections of 2 μl each applied with an interval of 3 min. The binding isotherm profile was obtained excluding the initial data point. The top panel depicts the calorimetric output during the injection of HS into the rTvAD1 solution. The bottom panel depicts the binding isotherm.