TABLE 2.

Comparison of the rates^a for extension and degradation of each primer-template by WT Pol and A987G Pol

P-T	Nucleoside	Enzyme (nM)	Extensionb(nM/min)		Degradationb (nM/min)
			WT	A987G	WT	A987G
T1	GCV-TP	30	48 ± 2	53 ± 3	30 ± 5	31 ± 3
T2 (N)	dCTP	10	30 ± 5	38 ± 5	20 ± 4	15 ± 5
T3 (N + 1)	dATP	10	6 ± 1	14 ± 2	NDc	NDc
T4 (N + 2)	dTTP	30	36 ± 1	72 ± 4	59 ± 3	57 ± 2

^aThe extension rates for incorporation of GCV-TP or the relevant dNTP indicated in each row and the degradation rates on each primer-template were determined by plotting the amount of extended or degraded primer-templates versus time. P-T, primer-template.

^bErrors are SEM generated from two independent replicates.

^cND, not detectable.