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. 2021 Feb 9;12(1):e03471-20. doi: 10.1128/mBio.03471-20

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Copyright © 2021 Fernandez et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

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FIG 5 — Loss of metacaspase genes delays conidial germination and subsequent formation of appressorium on hydrophobic surfaces. (A) Spores of WT KV1, ΔMomca1, ΔMomca2, ΔMomca1mca2, and ΔMomca1mca2-C were applied to artificial hydrophobic surfaces (coverslips). (B) At 3 and 5 h postinoculation (hpi), the rate of conidial germination was measured and compared with that of the WT. (C) At 3, 5, 8, 12, and 20 hpi, the percentage of appressorium development was measured and compared with that of the WT. Results are means from three independent biological measurements. A total of 150 spores were counted for each replicate. Images were taken at each time point. Error bars denote standard deviations. Asterisks indicate statistically significant differences (*, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001; two-way ANOVA with Tukey’s multiple-comparison test using GraphPad Prism 8). Scale bar is 10 μm.