FIG 6.

Impact of 2′ acetylation on ribosomal binding of apramycin. Crystal structure of apramycin bound to the ribosome (PDB ID 4AQY). Important interactions between N3 and the ribosome for recognition of the 2-DOS ring are indicated. Intramolecular interaction between N2′ and O5 is highlighted. Acetylation at position N3 creates steric clash with the phosphate backbone. Acetylation of the N2′ position is predicted to create an electronic clash with the negatively charged backbone of A1492 and G1491.