FIG 5.

Lack of colocalization in vivo between [π] and [Het-s] or [Φ] prions. (A) Micrographs of strains coexpressing HET-s-RFP ([Het-s] or [Het-s*] states, noted here as [s*] and [s]) and GFP-HELLP(214-271) ([π*] or [π] state). Note an absence of colocalization of the prion forms and the independent occurrence of [π] and [Het-s]. Bar, 5 μm. (B) Micrographs of strains coexpressing HELLF(209-277)-RFP ([Φ] or [Φ*] state) and GFP-HELLP(214-271) ([π*] or [π] state). Note an absence of colocalization of the prion forms and the independent occurrence of [π] and [Φ] (bar, 5 μm).