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Primer's sequences of target (RASSF1A) and reference (ABL) genes
|
Primer's
name |
Primer's type | Primer's sequence | GC content | TM (°C) | Length (bp) | PCR product size (bp) |
|---|---|---|---|---|---|---|
| RASSF1A | F | 5'- CCCTGCTGCGAAAGTTCTTG-3' | 55 | 60.5 | 20 | 60 |
| RASSF1A | R | 5'- CGCGCTCAAAGAGTGCAAA-3' | 52.6 | 57.3 | 19 | |
| ABL | F | 5'- TGGAGATAACACTCTAAGCATAACTAAAGG-3' | 36.67 | 60.8 | 30 | 124 |
| ABL | R | 5'- GATGTAGTTGCTTGGGACCCA-3' | 52.38 | 60 | 21 |
F: forward, R: reverse, TM: temperature of melting
