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Graphical abstract representing the cells preparation for fluorescent detection of intracellular reactive oxygen species using the 2′,7′-dichlorofluorescein diacetate (DCFDA) dye. CP (CP) and hydrogen peroxide were chosen as a positive control and HEK293 cells were pre- and post-treated with 0.2, 2 and 10 μM of 4-HCH.
