Fig. 5. FRAP analysis of the assemblies formed between hTNKS2 ARC1–5 and TBP-CTPR proteins. (A) FRAP analysis was performed on seven individual Regions of Interest (ROI) selected within macromolecular assemblies induced by eGFP-tagged hTNKS2 ARC1–5 in complex with 3TBP-CTPR6 (orange) or 3TBP-CTPR6-foldon (green). HEK293T cells were bleached in the ROI and fluorescence recovery of eGFP-tagged hTNKS2 ARC1–5 was monitored over 90 seconds. (B) Representative ROI selected within three independent macromolecular assemblies are shown before, during and post bleaching.
