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Effects of TGFBR2 on apoptosis and inflammation in LPS-induced HK2 cells. HK2 cells were treated with LPS or/and transfected with si-TGFBR2. (a) The protein level of TGFBR2 was measured by western blot analysis. (b) Cell apoptosis was detected by the flow cytometry. (c) The protein levels of Bax, Bcl-2, and cleaved caspase 3 were analyzed by western blot. (d and e) ELISA kit was used to determine the production of pro-inflammatory cytokines (TNF-α and IL-2). *P < 0.05.
