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. 2021 Feb 4;16(1):274–283. doi: 10.1515/med-2021-0222

Figure 4.

Figure 4

miR-128-3p directly targeted TGFBR2 in HK2 cells. (a) The putative binding sites of miR-128-3p and TGFBR2 were provided by TargetScan. (b) Luciferase activity was measured in HK2 cells co-transfected with TGFBR2-wt or TGFBR2-mut and miR-128-3p or NC by dual-luciferase reporter assay. (c) RIP assay was performed to detect TGFBR2 enrichment level in HK2 cells transfected with miR-128-3p or NC. *P < 0.05. (d) The protein level of TGFBR2 was measured in HK2 cells transfected with miR-128-3p, NC, anti-miR-128-3p, or anti-NC by western blot analysis. *P < 0.05.