FIG 1.

Schematic representation of the slr0977 (kpsM) genomic context in Synechocystis sp. PCC 6803 and the generation of the slr0977 knockout mutant by double homologous recombination. slr0982, slr0983, slr0984, and slr0985 are annotated as rfbBFG and rfbC, while slr1610 is annotated as encoding a methyltransferase (3). The remaining ORFs encode hypothetical proteins of unknown function. The transcriptional unit and transcription start site are annotated according to Kopf et al. (27). The predicted terminator was found using the FindTerm algorithm (Softberry); the insertion of the antibiotic cassette did not alter the prediction.