FIG 6.

Exoproteomes of Synechocystis sp. PCC 6803 wild-type (wt), kpsM mutant, and complemented mutant strains. (A) Analysis of the proteins separated by SDS-PAGE and stained with Roti-Blue. Bands highlighted with an asterisk were observed across at least three biological replicates and excised for protein identification. (B) Analysis of the glycoproteins by SDS-PAGE followed by staining with a glycoprotein staining kit (Pierce). Arrowheads indicate glycosylation differences in the PilA component. Sample loading was normalized to each culture cell density (OD₇₃₀), volume of cell-free medium concentrated, and concentration factor. M, NZYColour protein marker II (NZYTech).