TABLE 1.
Diffusion constants and percentages of static and mobile molecule fractions
Strain | No. of cells | No. of tracks | Da | D1b | F1c | D2d | F2e |
---|---|---|---|---|---|---|---|
Nontreated | |||||||
DnaE-mV | 136 | 6,058 | 0.032 ± 0.004 | 0.053 ± 0.005 | 58 ± 2.2 | 0.75 ± 0.014 | 42 ± 1.6 |
DnaC-mV | 122 | 5,303 | 0.039 ± 0.005 | 0.093 ± 0.004 | 81 ± 1.7 | 0.82 ± 0.025 | 19 ± 1.3 |
DnaG-mV | 150 | 5,893 | 0.153 ± 0.007 | 0.100 ± 0.005 | 46 ± 1.9 | 0.88 ± 0.020 | 54 ± 1.6 |
MMC treated | |||||||
DnaE-mV | 136 | 5,275 | 0.027 ± 0.005 | 0.053 ± 0.005 | 63 ± 1.7 | 0.75 ± 0.014 | 37 ± 2.2 |
DnaC-mV | 134 | 5,139 | 0.107 ± 0.005 | 0.093 ± 0.004 | 42 ± 1.6 | 0.82 ± 0.025 | 58 ± 1.8 |
DnaG-mV | 135 | 7,097 | 0.174 ± 0.007 | 0.100 ± 0.005 | 29 ± 1.4 | 0.88 ± 0.020 | 71 ± 1.5 |
HPUra treated | |||||||
DnaE-mV | 121 | 4,775 | 0.093 ± 0.007 | 0.053 ± 0.005 | 49 ± 2.3 | 0.75 ± 0.014 | 51 ± 1.2 |
DnaC-mV | 134 | 5,135 | 0.078 ± 0.005 | 0.093 ± 0.004 | 42 ± 1.5 | 0.82 ± 0.025 | 58 ± 1.5 |
DnaG-mV | 110 | 5,526 | 0.109 ± 0.007 | 0.100 ± 0.005 | 81 ± 1.6 | 0.88 ± 0.020 | 19 ± 1.1 |
SHX treated | |||||||
DnaE-mV | 158 | 5,067 | 0.220 ± 0.007 | 0.053 ± 0.005 | 12 ± 2.4 | 0.75 ± 0.014 | 88 ± 1.9 |
DnaC-mV | 171 | 6,690 | 0.187 ± 0.003 | 0.093 ± 0.004 | 13 ± 1.5 | 0.82 ± 0.025 | 87 ± 1.4 |
DnaG-mV | 157 | 6,937 | 0.230 ± 0.005 | 0.100 ± 0.005 | 12 ± 1.6 | 0.88 ± 0.020 | 88 ± 1.2 |
D, average diffusion constant of all molecules (μm2·s−1).
D1, diffusion constant of static fraction (μm2·s−1).
F1, percentage of static molecules.
D2, diffusion constant of mobile fraction (μm2·s−1).
F2, percentage of mobile molecules.