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. 2021 Jan 27;6(1):e01076-20. doi: 10.1128/mSphere.01076-20

TABLE 4.

Conjugative transfer-related genes identified in pAC1530 and pAC1633-1 compared to their corresponding genes in pA297-3

Gene Product size pAC1530a (aa) Product size pA297-3b (aa) % Protein identityd Possible functionc
Region 1
    traW 378 377 75 (282/376) Lipoprotein
    traY 902 882 66 (526/729) Integral membrane protein
    trbA 445 456 62 (273/443) Formation of thick pilus/DNA transfer
    traJ 408 429 62 (262/410) DNA-binding protein
    traI 277 272 69 (192/279) Lipoprotein
    traH 156 156 66 (105/158) Lipoprotein
    traM 232 238 60 (133/231) Formation of thick pilus/DNA transfer
    traN 367 376 63 (237/379) Signal peptide
    traO 541 543 50 (252/501) Formation of thick pilus/DNA transfer
    icmJe 255 252 60 (149/250)
    traU 1088 1090 70 (754/1084) Nucleotide-binding protein
    trbC 913 912 61 (569/912) Nucleotide-binding protein
Region 2
    traC 632 616 49 (305/619) Inner membrane complex ATPase
    trbNf 272 275 50 (142/266) Major pilus subunit
    traB 750 753 60 (451/753) Outer membrane complex
a

GenBank accession no. CP045561.1.

b

GenBank accession no. KU744946.1 (Hamidian et al., 2016) (67).

c

Predicted function as listed in Hamidian et al. (2016) (67) and Christie (2016) (91).

d

Numbers in parentheses indicate amino acid identities.

e

icmJ as annotated by PROKKA; shared 60% amino acid sequence identity with HPG66_RS00900 of pA297-3, which was annotated as hypothetical; low sequence identities (42%; 18/43) with IcmJ of Legionella pneumophila (accession no. CAH11669.1).

f

trbN was annotated by Hamidian et al. (2016) (67) for pA297-3 and pD46-4 (Nigro and Hall, 2017) (70); no detectable similarities with other conjugal proteins.