. 2021 Jan 27;6(1):e01227-20. doi: 10.1128/mSphere.01227-20

TABLE 2.

Primary and secondary antibodies used for flow cytometry analysis

Primary antibody	Secondary antibody (source)	Dilution
Bovine anti-OmpA	Goat anti-bovine IgG(H+L)-Alexa Fluor 647 (Jackson Immunoresearch)	1/200
Bovine serum	Sheep anti-bovine IgM-FITC (Bio-Rad Antibodies)	1/50
	Sheep anti-bovine IgG₂-FITC (Bio-Rad Antibodies)	1/50
Mouse MAb to bovine C3 (clone MD3)	Rabbit F(ab′)2 a-mouse-IgG-RPE (Bio-Rad Antibodies)	1/100
Rabbit serum to type 1 fimbriae	Donkey anti-rabbit IgG-RPE (Jackson Immunoresearch)	1/200