TABLE 1.
Plasmids used in this study
| Plasmid | Description | Resistance phenotypea | Reference |
|---|---|---|---|
| pRMH977 | IS26 in pUC19 | Ap | 6 |
| pRMH1011 | IS1006 in pUC19b,c | Ap | This study |
| pRMH1012 | IS1008 in pUC19c,d | Ap | This study |
| pRMH1013 | IS1006/1008 in pUC19c,e | Ap | This study |
| pRMH1015 | IS26 with internal catA1 gene in pUC19c,f | Ap Cm | C. J. Harmer and R. M. Hall, submitted for publication |
| pRMH762 | IS26 in pUC19 | Ap | 6 |
| pRMH962 | IS26-FS-L in pUC19 | Ap | 6 |
| R388 | IncW plasmid | Su Tp | 22 |
| R388::IS26 | IS26 in R388 | Su Tp | 6 |
| R388::IS26-2 | IS26 in R388g | Su Tp | This study |
| R388::IS26-FS-R | R388::IS26 frameshift mutant | Su Tp | 6 |
| R388::IS1006 | IS1006 in R388b,g | Su Tp | This study |
| R388::IS1008 | IS1008 in R388d,g | Su Tp | This study |
| R388::IS1006/1008 | IS1006/1008 in R388e,g | Su Tp | This study |
a
Ap, ampicillin; Su, sulfamethoxazole; Tp, trimethoprim.
b
Bases 39531 to 40583 from pD36-4 (GenBank accession no. CP012956).
c
Insert cloned into the pUC19 BamHI site by Gibson assembly.
d
Bases 88655 to 90375 from pA297-3 (GenBank accession no. KU744946).
e
Bases 42241 to 43233 from pJ9-3 (GenBank accession no. CP041590).
f
Synthetic construct, equivalent to pRMH977, with bases 133211 to 133909 of pRMH760 (GenBank accession no. KF976462) containing the catA1 gene and its natural promoter inserted between bases 775 and 776 of IS26.
g
Insert cloned between the two R388 HindIII sites by Gibson assembly.