TABLE 2.

Frequency of cointegrate formation

ISb	Target	Cointegration frequencya
		Range	Mean (SD)
Untargeted copy-in
IS26	R388	4.8 × 10−7 to 5.4 × 10−7	5.1 × 10−7 (2.9 × 10−8)
IS1006	R388	3.2 × 10−7 to 8.0 × 10−7	5.9 × 10−7 (2.5 × 10−7)
IS1008	R388	1.0 × 10−7 to 4.7 × 10−7	3.4 × 10−7 (2.1 × 10−7)
IS1006/1008	R388	5.8 × 10−7 to 8.7 × 10−7	6.8 × 10−7 (1.7 × 10−7)
Targeted conservative
IS26	R388::IS26	4.6 × 10−4 to 7.2 × 10−4	6.1 × 10−4 (1.4 × 10−4)
IS26	R388::IS26-2	4.7 × 10−4 to 9.7 × 10−4	8.0 × 10−4 (2.9 × 10−4)
IS1006	R388::IS1006	2.4 × 10−5 to 7.7 × 10−5	5.1 × 10−5 (2.4 × 10−5)
IS1008	R388::IS1008	1.0 × 10−5 to 5.1 × 10−5	3.1 × 10−5 (2.1 × 10−5)
IS1006/1008	R388:: IS1006/1008	3.0 × 10−5 to 7.8 × 10−5	4.7 × 10−5 (2.7 × 10−5)

^aFrequency measured as cointegrates per transconjugant. Three independent determinations were made per experiment.

^bIS in the pUC19 backbone.