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. 2021 Feb 16;21:111. doi: 10.1186/s12935-021-01811-4

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — circMED13L_012 modulates miR-433-3p expression. a Bioinformatic prediction of circMED13L_012 interacting miRNAs using Circinteractome and Starbase online database platform. b–d Association of circMED13L_012 expression level and miR-433-3p, miR-503-3p, miR-330-3p expression level in NSCLC tumor samples. e Design of wildtype and mutated circMED13L_012 vector and predicted binding location with miR-433-3p. f, g Dual luciferase reporter gene assay via wildtype or mutated circMED13L_012 vector transfection with or without treatment of sh-circMED13L_012 to validate the binding of miR-433-3p with wildtype circMED13L_012. h, i. RNA immunoprecipitation assay to detect the binding of circMED13L_012 and miR-433-3p with anti-Ago2 antibody, in comparison with control IgG antibody in A549 and NCI-H1299 tumor cells. j. qRT-PCR evaluation of the impact of circMED13L_012 overexpression and silencing on expression level of miR-433-3p in NCI-H1299 and A549 cells.