Fig. 6.

Function identification of circMED13L_012-miR-433-3p–MAPK8 axis in NSCLC cells. a Western Blot and qRT-PCR validation of the effects of MAPK8 specific siRNAs treatment on the protein and mRNA level of MAPK8 in A549 cells. b Cell proliferation analysis on NCI-H1299 cells transfected by circMED13L_12 overexpression vector, with or without MAPK8 siRNA. c–e Cell survivability test of NCI-H1299 and A549 cells which were transfected by circMED13L_12 overexpression vector, with or without MAPK8 siRNA. Cell groups were challenged by three different chemo-agents with a range of dosages respectively, i.e. docetaxel, doxorubicin and gefitinib. f Western blot analysis on A549 cells transfected by circMED13L_12 overexpression vector, with or without miR-433-3p mimics. g qRT-PCR study on A549 cells transfected by circMED13L_12 overexpression vector, with or without miR-433-3p mimics. h Western blot analysis on NCI-H1299 cells transfected by sh-circMED13L_12 vector, with or without miR-433-3p mimics. i qRT-PCR study on NCI-H1299 cells transfected by sh-circMED13L_12 vector, with or without miR-433-3p mimics