Table 1.
Target | Forward primer | Reverse primer | Annealing temp (°C) |
---|---|---|---|
RPL13A* | 5ʹ-cctggaggagaagaggaaagaga-3ʹ | 5ʹ-ttgaggacctctgtgtatttgtcaa-3ʹ | 57.1 |
RPL19* | 5ʹ-tgaggagaatgaggattt-3ʹ | 5ʹ-gtacaggctgtgatacat-3ʹ | 54.1 |
H3F3B* | 5ʹ-cattatctaggtccttgta-3ʹ | 5ʹ-aatacagcactattatgg-3ʹ | 54.1 |
B2M* | 5ʹ-gactggtctttctatctct-3ʹ | 5ʹ-cttcaaacctccatgatg-3ʹ | 55 |
HPRT1* | 5ʹ-aagcctaagatgagagtt-3ʹ | 5ʹ-ccacagaactagaacattg-3ʹ | 55.8 |
GAPDH* | 5ʹ-cctcaagatcatcagcaat-3ʹ | 5ʹ-ttccacgataccaaagtt-3ʹ | 55 |
TUBB3* | 5ʹ-ggcatctcttgagaacaa-3ʹ | 5ʹ-gacctgtacctgtctctc-3ʹ | 55 |
SV2A | 5ʹ-agcatgacgaaggtgaat-3ʹ | 5ʹ-gtagctgtgacgtgttgta-3ʹ | 58.9 |
SV2B | 5ʹ-ttcaggcactaaggtctct-3ʹ | 5ʹ-aaggaagcgattctcaatgtt-3ʹ | 57.1 |
SV2C | 5ʹ-ctggagcgttctgttttg-3ʹ | 5ʹ-aattttcacagcctcctttc-3ʹ | 57.1 |
DAT | 5ʹ-gccgtggtcttcttcatcat-3ʹ | 5ʹ-aacagggacaggaggaaggt-3ʹ | 58.1 |
CHAT | 5ʹ-agatgttctgctgctatg-3ʹ | 5ʹ-gaaaaggatggtctctgg-3ʹ | 55.8 |
NET | 5ʹ-agcttttggtggtcagatgg-3ʹ | 5ʹ-cagagcaagagcagcatgag-3ʹ | 61.4 |
SNAP25 | 5ʹ-taacacttcttacgcaatg-3ʹ | 5ʹ-gaacgaaccaactgatt-3ʹ | 57.1 |
FGFR3 | 5ʹ-agactgaaattacgggta-3ʹ | 5ʹ-ccatatacacagcatctatt-3ʹ | 55.8 |
Primers used for housekeeping genes are marked with an asterisk (*)