Fig.6.

WNT/β-catenin signaling pathway is involved in AA-induced HK2 cells injury. HK2 cells were treated with β-catenin shRNA or Scramble shRNA in the presence with 10 µmol/L AA. a real-time PCR analysis of Caspase1, IL-1β, E-cadherin, Vimentin, PAI-1, and MMP9 mRNA levels in HK2 cells. b Representative immunofluorescence images of E-cadherin and Vimentin in HK2 cells. Scale bar = 50 μm. c Representative migration results of HK2 cells. Scale bar = 50 μm. Data are shown as mean ± s.d.*P < 0.05 vs Control, ^# P < 0.05 vs AA. All experiments were performed in triplicate