Fig. 7.

WNT/β-catenin pathway is required for SIK1 mediated HK2 cells injury induced by AA. HK-2 cells were co-transfected with SIK1 and β-catenin shRNA or transfected with SIK1 or β-catenin shRNA alone, and then treated with 10 µmol/L AA for 72 h. a real-time PCR analysis of Caspase1, COL1, E-cadherin, and Vimentin expression. b Western blot analysis of Caspase1/p20/p10, E-cadherin, and Vimentin expression. Data are shown as mean ± s.d.*P < 0.05 vs Control, ^#P < 0.05 vs SIK1 shRNA, ^&P < 0.05 vs β-catenin shRNA. All experiments were performed in triplicate