Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Feb 16;54:5. doi: 10.1186/s40659-021-00329-3

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

PMC Copyright notice

Fig. 6 — mTBI increases exocyst complex assembly and interaction between Exo70 and GluN2B in the PSD fraction. Two-month-old male mice were subjected to mTBI and hippocampal PSD/nonPSD fractions were obtained. 50 and 300 µg of PSD (a) and nonPSD (c) protein samples, respectively, were immunoprecipitated using a specific Exo70 polyclonal antibody. Beads were recovered by brief centrifugation. Samples were resolved in 6% SDS-PAGE and transferred to PVDF membranes. Signal intensity was normalized with Exo70 band intensity. Both hippocampi of each mouse were used. Membranes were stripped and tested again with the indicated antibodies. b, d GluN2A, GluN2B, GluR1, GluR2, Sec6, and Sec10 densitometric analysis from PSD and nonPSD immunoprecipitations. The analysis shows the assembly level of the exocyst complex and Exo70-GluN2B increased interaction after mTBI. Values represent means ± SEM, n = 3 mice per experimental group. Statistical differences were determined by an unpaired t-test comparing Sham and mTBI. *p < 0.05