Table 1.
Primers employed in the real-time RT-PCR analysis
| Genes | Sequences | Tm (°C) |
|---|---|---|
| Aggrecan |
Forward: 5′-CCATCTCTACACGCTACACCC-3′ Reverse: 5′-TTGTCTCCATAGCAGCCTTCC-3’ |
60 °C |
| Type-II collagen |
Forward: 5’-CGTCTACCCCAATCCAGCAAA-3′ Reverse: 5′-AGCAGGCGTAGGAAGGTCAT-3’ |
60 °C |
| SOX9 |
Forward: 5’-CACACTCCTCCTCCGGCATGA-3′ Reverse: 5′-GCGGAAGTCGATAGGGGGCT-3’ |
63 °C |
| Integrin β1 |
Forward: 5’-GCGAGTGTGGTGTCTGTAAGT-3′ Reverse: 5′-CATTCCTGTGTGCATGTGTCTT-3’ |
60 °C |
| GAPDH |
Forward: 5’-AGCCAAAAGGGTCATCATCTCT-3′ Reverse: 5′-AGTCCTTCCACGATACCAAAGT-3’ |
60 °C |